Hi Amedee,
Regularization is an intrinsic part of the reconstruction algorithm within
the classification procedure, so the answer to your question is no. You
could however use relion_reconstruct of the particles in their optimal
orientations. Alternatively, run auto-refine on each class.
HTH,
S
> Hello Everyone,
>
> Is there a way to get unregularized (unfiltered) reconstructions at the
> end of a classification iteration? I don’t see an option for it, even
> though we can get regularized half-set maps.
>
> By advance, many thanks,
>
> Amedee
>
>
>
> --
> --
> Amedee des Georges
> Frank lab
> Howard Hughes Medical Institute
> Dept. of Biochemistry and Molecular Biophysics
> 650 W 168th St, P&S Black Building room 221
> New York, NY 10032
> (T) 212 305-9527
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> Google Scholar Profile
>
>
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
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