Hi Charles,
I don't know what multiscale does. Probably the right thing. If the
anomalous signal is weaker after scaling of multiple datasets,
non-isomorphism might be at fault. Try Blend to scale your datasets.
Blend is part of ccp4 and gives good graphical diagnostic feedback.
Andreas
On 01/03/2015 4:20, CPMAS Chen wrote:
> Dear CCP4 users,
>
> Recently, I got some datasets with weak anomalous Br signal. I tried to
> merge them according to Q. Liu et al Science 336, p1033 (2012). I am
> using the script [log in to unmask] The merged dataset has WEAKER
> anomalous signals.
>
> Liu et al used SCALA for scaling and merging while multiscale@SSRL using
> AIMLESS. Should this cause such a difference? The SCALA@SSRL has a
> limitation on the number of frames it can process. So I cannot directly
> check if this caused the difference.
>
> Any suggestions?
>
> Thanks!
>
> Charles
>
> --
>
> ***************************************************
>
> Charles Chen
>
> Research Associate
>
> University of Pittsburgh School of Medicine
>
> Department of Anesthesiology
>
> ******************************************************
>
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