Have you tried crystallising in microbatch format, i.e. under oil?
I've had success with this method for exactly the problem you describe.
Regards.
Antony
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> On 25 Feb 2015, at 08:39, Ulrike Demmer <[log in to unmask]> wrote:
>
> Dear crystallographers,
>
> I am trying to crystallize a soluble protein which tends to form aggregates. The crystallization condition is 20% PEG 3350 + 0,2 M Na-Formate. During the crstallization process a thick skin is formed on top of the sitting-drops. As well the crystals are buried in precipitate. Before I start harvesting I try to remove the skin but still it is hardly possible to get any crystals out of these drops.
>
> Any suggestions how to avoid the formation of skin on crystallization drops ?
>
> Cheers,
>
> Ulrike
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