Just a little mother liquor. I am seeing what SO4 does in refinement right now.
JPK
-----Original Message-----
From: Boaz Shaanan [mailto:[log in to unmask]]
Sent: Monday, February 23, 2015 2:50 PM
To: Keller, Jacob; [log in to unmask]
Subject: RE: [ccp4bb] Blob Game
Boaz Shaanan, Ph.D.
Dept. of Life Sciences
Ben-Gurion University of the Negev
Beer-Sheva 84105
Israel
E-mail: [log in to unmask]
Phone: 972-8-647-2220 Skype: boaz.shaanan
Fax: 972-8-647-2992 or 972-8-646-1710
________________________________________
From: CCP4 bulletin board [[log in to unmask]] on behalf of Keller, Jacob [[log in to unmask]]
Sent: Monday, February 23, 2015 8:53 PM
To: [log in to unmask]
Subject: Re: [ccp4bb] Blob Game
>Ethanol? You weren't setting up drops with liquor on your breath, were
>you I can't remember.
Ahah, so you did have some liquor. Good.
Seriously, if there is something below the blob in the direction that you sent us and could be joined with the peak, couldn't an SO-4 fit in there somehow? It's too detailed for water at this resolution, isn't it?
Boaz
JPK
On 02/23/2015 12:20 PM, Keller, Jacob wrote:
> Dear Crystallographers,
>
> I've got a strange blob hanging off an arginine--see attached. Any ideas? Nothing weird in the prep; cryst+prot conditions: NH4SO4, TRIS/HEPES, CaCl2, NaCl, Li2SO4. There are really no other side chains which could be close enough, and there's nothing in the anomalous LLG map.
>
> ???
>
> JPK
>
> *******************************************
> Jacob Pearson Keller, PhD
> Looger Lab/HHMI Janelia Research Campus
> 19700 Helix Dr, Ashburn, VA 20147
> email: [log in to unmask]
> *******************************************
>
>
|