A little more info:
-Resolution is 1.75 Ang at edge of detector, but I/sig was still ~3; pushing integration to corners gives 1.66 Ang, but completeness is horrible. The home setup was at the limit (without 2 theta.)
-Data are horribly twinned with fractions of 58/42%, spacegroup is P3212. Besides that, though, the data are really excellent.
-Peak is 6.40 rmsd in Refmac-generated difference map.
-Distance from blob-peak to Arg-N is 3.43 Ang, but the peak is obviously not spherical.
JPK
-----Original Message-----
From: Keller, Jacob
Sent: Monday, February 23, 2015 12:34 PM
To: Keller, Jacob; [log in to unmask]
Subject: RE: Blob Game
...And the cryoprotectant was simply the Li/NH4SO4 mother liquor. Also maybe relevant: the crystals were pretty old, a year or so.
JPK
-----Original Message-----
From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of Keller, Jacob
Sent: Monday, February 23, 2015 12:21 PM
To: [log in to unmask]
Subject: [ccp4bb] Blob Game
Dear Crystallographers,
I've got a strange blob hanging off an arginine--see attached. Any ideas? Nothing weird in the prep; cryst+prot conditions: NH4SO4, TRIS/HEPES, CaCl2, NaCl, Li2SO4. There are really no other side chains which could be close enough, and there's nothing in the anomalous LLG map.
???
JPK
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Jacob Pearson Keller, PhD
Looger Lab/HHMI Janelia Research Campus
19700 Helix Dr, Ashburn, VA 20147
email: [log in to unmask]
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