Apparently, the iitial model has little handedness, so that the refinement
can converge onto either of the hands. Nothing to worry about, just keep
an eye on the correct one.
Mike, indeed: less stringent filtering will probably maintain more
handedness in the initial model.
HTH,
S
> This has happened to me twice. I found no clean solution other than to
> filter my initial model less strongly.
>
> mike
>
>> On Jan 20, 2015, at 3:22 PM, Tat Cheng <[log in to unmask]> wrote:
>>
>> Hi Dr Scheres and all,
>>
>> I noticed that there is a change of handedness in 3D classification
>> resulting models from the initial model. The data is 2x binned, given 25
>> cycles and T=4, initial model filtered to 60A. When I tried to do
>> another run of 3D classification using particles from a single class
>> from the first 3D classification run1, using the corresponding
>> hand-changed model, and this time the data is unbinned, but the
>> handedness doesn't change.
>> I can flip the resulting models easily to get the right hand, but I
>> wonder has anybody seen this before?
>> Thanks a lot.
>>
>> Tat
>
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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