Hi Mike,
This may also be due to trouble at the last combination step. Try the
option I recommended to Giulia before.
HTH, S
> Hello Relion users,
>
> Has anyone seen anything like this? (See attached image) The image shows
> the final half volumes (_unfil.mrc) from 3D auto refine after it performs
> the final reconstruction step using unbinned data. While one of the half
> volumes (right) looks fine, one of the half volumes (left) has strange
> artifacts that I’ve never seen before.
>
> Has anyone seen this? I’m not sure where this could be coming from.
>
> Info on the data: I am reanalyzing the published 80S ribosome dataset from
> Bai et al. eLife 2013. Box size = 240 x 240 x 240; Pixel size 1.77 A/pix.
>
> Attached image: Raw, unbinned half volumes from the final reconstruction
> step in Relion-1.3.
>
> Thanks for your feedback!
> Mike
>
> ____________________
> Michael A. Cianfrocco, Ph.D.
> Post-doctoral fellow of the Damon Runyon Cancer Research Foundation
> Reck-Peterson & Leschziner Labs
> Depts. of Cell Biology Harvard Medical School & Molecular and Cellular
> Biology Harvard University
> Boston, MA, USA 02115
>
> [cid:18506C4A-CB2C-4355-AE1A-1D0FB4C61ED9@cable.rcn.com]
>
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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