Hi Ludo,
Your B-factor plot is a bit noisy, this is often the case for not-so-high
resolution maps, as the Guinier plot has few points to fit through. What
you could do is fit continuous curves through both the B-factor plot and
the intercept-plot (in the same bfactors.star file) using whatever you
like (even a pencil on a printed plot is ok) ;-) Then you replce the
values in the _bfactors.star file with the fitted ones, and you delete the
shiny.star file in the project directpry (or you move it somewhere safe),
and finally you re-run the polishing using the "continue an old run"
option on the GUI. The program will recognize the half-maps are all there,
as well as your bfactors.star plots and will use these instead of starting
from scratch again. The program will therefore only re-make the shiny
particles, now with the better weights.
HTH,
S
PS: Sorry for the delay. I've decided not to answer ccpem messages
immediately anymore. They were giving too much distractions during the
day. Instead, I'll do them once every few days from now.
> Hi all,
> I have processed some movie data (51 frames per movie) and plotting the
> b-factors and per-frame relative weights I noticed 2 non-consecuitive
> frames having the lowest b-factors and contributing to the highest
> resolution data in the weighting scheme.
>
>
>
> Those frames are like 37 and 46.
> Is that normal?
> A silly question would be: can I only use those 2 frames for further 3d
> refinement?
> Thanks,
> Ludo
>
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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