Hi everyone
I processed my unbinned data until particle extraction step.
Then I decide to do a binning of 5, due to the limitation of computation resource.
The magnified pixel size in the GUI was 1.06 and the particle mask diameter was 320.
so, I set Re-scaled size (pixels) in the GUI to 64.
In order to do particle sorting, I also rescale my reference by relion_image_handler with
"--angpix 1.06 --rescale_angpix 5.30 --new_box 64"
My questions are:
1.) am I on the right track so far ?
2.) after all these steps, in the gui should I changed the The magnified pixel size in the GUI to 5.3 or leave it as 1.06?
I could do particle sorting in both cases. The two sortings look a bit different but both seems ok.
Thanks.
Chia
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