Dear Jeorge,
I can’t see anything in that output saying whether the systematic absences that would allow you to detect screw axes were covered in your data collection.
In any case, it’s not necessary to reintegrate. All that happens when going from P222 to any one of the other 7 possible orthorhombic space groups is that some of the reflections will become systematically absent so they will be discarded, but Phaser will do that internally when testing all the space groups.
Best wishes,
Randy Read
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Randy J. Read
Department of Haematology, University of Cambridge
Cambridge Institute for Medical Research Tel: +44 1223 336500
Wellcome Trust/MRC Building Fax: +44 1223 336827
Hills Road E-mail: [log in to unmask]
Cambridge CB2 0XY, U.K. www-structmed.cimr.cam.ac.uk
On 26 Jan 2015, at 04:46, jeorgemarley thomas <[log in to unmask]> wrote:
> Hello Dr. Randy
> Here is the IDXREF.LP I got in which, on the basis of quality of fit, I went for this space group well I would also try for the other screw axes. So should I Integrate the data from beginning with all possible screw axes of orthogonal space group? I am attaching the IDXREF.LP screen shot here.
>
> Jeorge
>
> On Sun, Jan 25, 2015 at 5:00 PM, Roger Rowlett <[log in to unmask]> wrote:
> Did you search all 8 possibilities of screw axes, e.g. P2221, P21212, P212121, etc?
>
> Roger Rowlett
>
> On Jan 25, 2015 4:50 AM, "jeorgemarley thomas" <[log in to unmask]> wrote:
> Hi, I have processed the data using XDS and space group found to be P 2 2 2 (16) and I used the phaser MR for first phase determination. The model I have used has has more than 70 % sequence identity, when I run the phaser I got the message which I have attached here. And only sum. file I got as an output. Does any one have suggestion what should I do ? I would highly appreciate your kind suggestions. Thank you in advance.
>
>
>
> <IDXREF.PNG><SpaceG.PNG>
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