Hi Reza,
1.0 and 1.25 M citrate are potential cryo solutions. They still had faint ice rings present but were not dominant. It is also difficult to make a solution more concentrated then 1.75 M citrate. Hope this helps.
-Adam
> On Dec 1, 2014, at 10:59 AM, Reza Khayat <[log in to unmask]> wrote:
>
> Hi,
>
> Has anyone used citrate as the sole cryoprotectant? If so,
> what concentration was needed?
>
> Best wishes,
> Reza
>
> Reza Khayat, PhD
> Assistant Professor
> The City College of New York
> Department of Chemistry, MR-1135
> 160 Convent Avenue
> New York, NY 10031
> Tel. (212) 650-6070
> www.khayatlab.org
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