Dear Mani,
You could run a refinement in C1, and then symmetry the 2 unfil.mrc maps
prior to post-processing. Not sure what will happen to the density in the
corners (it will depend on the program you use for symmetrisation, but it
will probably somewhat affect your FSC curve), but overall things should
go more-or-less as you want.
HTH, S
> Hi Sjors and relion users,
>
> Is it possible impose the sym only in the last round of relion_refine?
>
> with kind regards
>
> Mani.
>
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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