Dear Manjula,
Unless Thrombin requires BSA for functioning (I never used Thrombin) you
could run a size exclusion column with the Thrombin solution prior to
using it. Or you could by it from a different vendor.
Regards,
Tim
On 10/08/2014 03:27 PM, Manjula Ramu wrote:
> Hi all,
>
> My protein size is nearly the same size of BSA and it has His tag. If I
> have to cleave the tag I have to use Thrombin which is having huge amount
> of BSA in it. Now how will I separate this BSA from my protein.
>
> Thanks and Regards,
> Manjula R
> Research Scholar
> Department of Biophysics
> National Institute of Mental Health and Neurosciences
> Bengaluru-29, Karnataka
> INDIA
> E-mail: [log in to unmask]
> Mobile no:+91-9538553356
> http://www.nimhans.kar.nic.in/
> <https://www.nimhans.kar.nic.in/>
>
--
Dr Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen
GPG Key ID = A46BEE1A
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