Hi,
I would stay away from the Mem-PER kit since the detergents in there are possibly quite harsh (?). I doubt that detergents would cause lysis of yeast cell walls in any case.
There is a budget-friendly alternative for mechanical breaking of yeast and that is the bead beater, sold by biospec. You'll need 0.5 mm glass beads which they also sell. I think they are about £500. Works quite well.
Bert
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From: CCP4 bulletin board [[log in to unmask]] on behalf of Theresa Hsu [[log in to unmask]]
Sent: Wednesday, October 08, 2014 1:25 PM
To: [log in to unmask]
Subject: [ccp4bb] Yeast membrane protein extraction
Dear all
Would you have a good protocol for membrane protein extract from S. cerevisiae? We don't have French press in our lab. Is the Pierce Mem-PER reagent kit useful for proteins (10-12 transmembrane helices) that will be use for crystallization?
Thank you.
Theresa
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