Dear,
the conditions you list should equilibrate within a couple of days. A
growth time of three months could be a sign of some proteolytic reaction
happening. You might want to sequence the crystal and subclone the
fragment that actually crystallises.
Best,
Tim
On 10/31/2014 11:05 AM, Vijaykumar Pillalamarri wrote:
> Dear all,
>
> I am trying to crystallize a 30 kD protein. Protein crystals are formed
> after 3 months. The crystals are formed in the following condition:
> 0.01M Zinc sulphate
> 0.1M MES monohydrate pH 6.5
> 25% v/v PEG monomethyl ether 550
>
> Please suggest me how to grow these crystals faster.
>
> Thanking you
>
--
Dr Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen
GPG Key ID = A46BEE1A
|