Using mixed solvents is another approach:
Ciccone L., Tepshi L., Nencetti, S. & Stura E.A. (2014)
Transthyretin complexes with curcumin and bromo-estradiol: Evaluation of
solubilizing multicomponent mixtures New Biotech. 32:54–64
http://dx.doi.org/10.1016/j.nbt.2014.09.002
Volatile solvents are a problem when you try to harvest the crystals.
Enrico.
On Wed, 15 Oct 2014 20:35:33 +0200, Jurgen Bosch <[log in to unmask]> wrote:
> An alternative is to dissolve your compound in MeOH and dispense it
> either manually or via robot, let the plate sit sometime in the hood for
> faster evaporation and then add your protein + reservoir.
> Jürgen
> ......................
> Jürgen Bosch
> Johns Hopkins University
> Bloomberg School of Public Health
> Department of Biochemistry & Molecular Biology
> Johns Hopkins Malaria Research Institute
> 615 North Wolfe Street, W8708
> Baltimore, MD 21205
> Office: +1-410-614-4742<tel:%2B1-410-614-4742>
> Lab: +1-410-614-4894<tel:%2B1-410-614-4894>
> Fax: +1-410-955-2926<tel:%2B1-410-955-2926>
> http://lupo.jhsph.edu
>
> On Oct 15, 2014, at 5:18 PM, Keller, Jacob
> <[log in to unmask]<mailto:[log in to unmask]>> wrote:
>
> Since you mentioned EtOH, why not do this:
>
> -Make a tray with the appropriate mother liquors
> -Make a drop for each well containing mother liquor and
> high-concentration ligand in EtOH (you could vary the ratios here as
> needed.)
> -Equilibrate by vapor diffusion until EtOH all goes into the well soln
> (couple of hours at the most?)
> -Add protein to these drops
>
> You could skip right to the protein step if your protein doesn't mind
> EtOH at fairly high concentrations, and anyway it will be gone fairly
> quickly, esp at RT
>
> Jacob Keller
>
> ________________________________________
> From: CCP4 bulletin board
> [[log in to unmask]<mailto:[log in to unmask]>] on behalf of
> Monica Mittal
> [[log in to unmask]<mailto:[log in to unmask]>]
> Sent: Wednesday, October 15, 2014 2:13 PM
> To: [log in to unmask]<mailto:[log in to unmask]>
> Subject: [ccp4bb] crystallization with hydrophobic ligands
>
> Dear All
>
> Can anyone give suggestions for handling the solubility problem of
> highly hydrophobic compounds, during co-crystallization or inhibition
> assays?
> The ligands I am using are almost insoluble in aquous medium. In DMSO or
> 95% Ethanol, the solubility is higher.
> Besides crystallization, this solubility is also a hindrance for
> in-vitro or in-vivo assays requiring higher conc. of ligand.
>
> Thanks in advance !
> Monica
>
--
Enrico A. Stura D.Phil. (Oxon) , Tel: 33 (0)1 69 08 4302 Office
Room 19, Bat.152, Tel: 33 (0)1 69 08 9449 Lab
http://www-dsv.cea.fr/ibitecs/simopro/ltmb/cristallogenese
LTMB, SIMOPRO, IBiTec-S, CE Saclay, 91191 Gif-sur-Yvette, FRANCE
http://scholar.google.com/citations?hl=en&user=Kvm06WIoPAsC&pagesize=100&sortby=pubdate
http://www.chem.gla.ac.uk/protein/mirror/stura/index2.html
e-mail: [log in to unmask] Fax: 33 (0)1 69 08 90 71
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