Dear all,
I've encountered people who refuse to freeze cells and always lyse fresh
pellets. Better protein, they say. I've never had reason to do so
myself, or even to believe in their voodoo. Up until now, maybe.
My protein expresses well and is almost all in the soluble fraction in
an expression test from a fresh pellet. The large-scale expression from
the same pellet, now frozen and thawed, yielded 90% insoluble protein.
If it's the freezing that dooms the protein, I'm happy to redo the
fermentor run. Are there other examples out there of this?
Thanks.
Andreas
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Andreas Förster
Crystallization and X-ray Facility Manager
Centre for Structural Biology
Imperial College London
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