Well, Pharmacia (now, GE) used to sell such a guard column for FPLC
prior to Akta.
And I remember buying adaptors to be able to connect the guard column
and FPLC columns to an HPLC system.
So all the tools exist.
But keep in mind that a guard column volume must in all cases be kept
minimal to avoid sample dilution which can be deleterious to resolution
in size-exclusion chromatography.
Prior to FPLC with (or without) a guard column, I have always used an
Eppendorf microcentrifuge followed by 2u-filtering prior to injecting
the sample and have consistently observed that these remove most
aggregates if not all.
HTH,
Nadir Mrabet
Pr. Nadir T. Mrabet
Structural & Molecular Biochemistry
N-gere - INSERM U-954
University of Lorraine, Nancy
School of Sciences and Technologies
& School of Medicine
9, Avenue de la Foret de Haye, BP 184
54505 Vandoeuvre-les-Nancy Cedex
France
Phone: +33 (0)3.83.68.32.73
Fax: +33 (0)3.83.68.32.79
E-mail: Nadir.Mrabet <at> univ-lorraine.fr
Cell.: +33 (0)6.11.35.69.09
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On 16/09/2014 19:01, Christian Roth wrote:
> Hi Anita,
>
> never heared about Guard columns for Superdex columns. These guard
> columns are usually for HPLC columns (RP) or Silica High pressure
> gelfiltration columns like from Tosoh Bioscience and part of the
> acutal column itself, though replaceable.
>
> Christian
>
> Am 16.09.2014 09:29, schrieb Anita P:
>> Hi All,
>> Sorry for this off topic.
>>
>> I have heard that there are these little columns called guard columns
>> which can be attached to AKTA purifiers. These columns prevent the
>> incoming huge aggregates to be deposited and blocking of the gel
>> filtration columns.
>>
>> Can any one advice me regarding where to purchase these columns. I could
>> not find them in GE website. We have Superdex 16/60 on AKTA purifier.
>>
>> Thanks in advance.
>> Have a good day
>>
>> Anita
>
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