Dear All,
When I use relion to process a helical assembly, at the 3D classification step, I often don’t get an even distribution on the azimuth angle. Sometimes, I may get a relative even distribution early on, when I run more iterations, it would get worse and all the particles concentrate on two opposite orientations. All the other orientations don’t have many particles. Does anyone know what might cause the uneven azimuth angle distribution? Thank you very much.
Best
gongpu
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