Dear all,
I would like to remove two phospholipids bound to (actually into) a cytoplasmic protein. The protein was expressed in E. coli and the crystal structure revealed the presence of two co-purified phospholipids (most probably DPPE).
A web search gave me three methods to remove bound lipids:
- 1-butanol liquid–liquid extraction
- Lipidex 1000, VI column at 37°C
- HIC (aka Hydrophobic Interaction Chromatography) on a Phenyl HP column in presence of 1M ammonium sulphate
All are described in Velkov 2008 (http://www.sciencedirect.com/science/article/pii/S1570023208002390).
I assume these delipidation methods would also work for phospholipid; and I am more tempting by the last one, the milder one.
I would appreciate any comment or practical advice.
Best regards,
Lionel
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