Dear Wei,
The enzyme ribonucleotide reductase can, depending on organism and class, bind ATP as a substrate in the active site (c), as an allosteric regulator of substrate specificity at another site (s) and as an overall activity regulator at a third site (a)! It can also bind dATP at the second and third sites, but not as a substrate. It cannot bind to sites (c) and (s) at the same time although it could potentially bind to sites (s) and (a) simultaneously. Here is a review that you might find useful:
http://www.ncbi.nlm.nih.gov/pubmed/22050358
Best wishes
Derek
On 4 Mar 2014, at 04:48, Wei Shi <[log in to unmask]> wrote:
> Dear all,
> Does anyone happen to know examples of 2 ligands bind to a single protein / each monomer protein in 2 different ligand binding pockets?
> I know the following example:
> (1). phosphofructokinase, which binds ATP as both a ligand and a feedback inhibitor in different sites
> (2). 2 cAMP bound to each E. coli CAP monomer in the crystal structure.
>
> Does any of you know other examples? Thank you so much!
>
> Best,
> Wei
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