Itšs not just possible, but desirable! Therešs evidence that
conformational ensembles can be accessed in RT crystallography data, but
that these are frozen out in cryo data (aforementioned Fraser et al.,
Accessing protein conformational ensembles using room-temperature X-ray
crystallography. PNAS, 2011, vol. 108,
16247-16252). Additionally, these ensembles have been linked to functional
mechanisms (van den Bedem et al, Nat Meth (2013) 10:896-902) and to NMR
order parameters (Fenwick et al, PNAS 2014 111 (4) E445-E454).
We generally see a reduction of ~0.2-0.3A in resolution from cryo to RT.
And yes, Pilatus detectors help, but you can certainly get good data on
other detectors.
On 2/6/14, 1:51 AM, "Theresa Hsu" <[log in to unmask]> wrote:
>Dear crystallographers
>
>Just out of curiosity, is it possible to collect datasets from crystals
>at room temperature at synchrotron? Are fast detectors like Pilatus
>useful for this?
>
>Thank you.
>
>Theresa
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