Dear All,
Since 80% saturated Li2SO4 has not been mentioned, I will do so. It is a
good cryosalt and I have often used
it even without any buffer added.
see:
Vera, L., Stura, E. A. (2013) Strategies for protein cryocrystallography.
Crystal Growth & Design, e-print
http://pubs.acs.org/doi/full/10.1021/cg301531f
The problem with phosphate precipitants is the formation of salt crystals
in the cryoprotectant solution.
Cryoprotecting molecules that normally work well with high ionic strength
conditions do not always
work well with phosphates.
800 mM Sodium phosphate monobasic/1200 mM Potassium phosphate dibasic
is well matched by 80% saturated lithum sulphate in terms of ionic
strength.
Enrico.
On Fri, 07 Feb 2014 06:20:58 +0100, Tanner, John J.
<[log in to unmask]> wrote:
> Try L-proline. It works well with high ionic strength conditions:
>
> http://www.ncbi.nlm.nih.gov/pubmed/22868767
>
> Sent from Jack's iPad
>
> On Feb 6, 2014, at 10:40 PM, "Deepak Thankappan Nair"
> <[log in to unmask]<mailto:[log in to unmask]>> wrote:
>
> Hello,
> Does anybody know what would be a good cryoprotectant for the following
> condition:
> 800 mM Sodium phosphate monobasic/1200 mM Potassium phosphate dibasic
> 100 mM Sodium acetate/Aceticacid pH4.5
>
> Thanks
> Deepak
>
--
Enrico A. Stura D.Phil. (Oxon) , Tel: 33 (0)1 69 08 4302 Office
Room 19, Bat.152, Tel: 33 (0)1 69 08 9449 Lab
http://www-dsv.cea.fr/ibitecs/simopro/ltmb/cristallogenese
LTMB, SIMOPRO, IBiTec-S, CE Saclay, 91191 Gif-sur-Yvette, FRANCE
http://scholar.google.com/citations?hl=en&user=Kvm06WIoPAsC&pagesize=100&sortby=pubdate
http://www.chem.gla.ac.uk/protein/mirror/stura/index2.html
e-mail: [log in to unmask] Fax: 33 (0)1 69 08 90 71
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