Hi Wu,
As mentioned on this email list before: the ctf estimation of the tutorial
is not done with the pre-set parameters. I should have long ago repaired
this in the tar.gz, but there are already many copies of it out there, and
I am planning on writing a new tutorial later this Spring anyway.
But here I repeat: if you use the pre-set parameters the program will
window the micrographs (to get rid of the barcode that our prototype
Falcon-II gives. You then need to set the window size to a value smaller
than half the 950 pixels in the window, otherwise CTFFIND3 crashes. The
_win.mrc files are deleted as they are only temporally useful.
You can also run the tutorial without any problem if you don't window the
micrographs: just use -1 for the windowed size in the GUI. You will then
not get any _win.mrc files.
HTH, S
> Hello,
>
> I am running the test data set using relion. The files in Micrograph
> directory is 006.mrc, but when I try to run preprocess using ctffind3, the
> scripts generated
>
>
> #!/usr/bin/env csh
> /home/wuw3/relion-1.2/bin/ctffind3.exe > Micrographs/006_ctffind3.log <<
> EOF
> Micrographs/006_win.mrc
> Micrographs/006.ctf
> 2, 300, 0.1, 79096, 56
> 512, 50, 16, 5000, 50000, 2000
> EOF
>
> It tried to find 006_win.mrc which does not exist in directory Micrograph.
> Please let me know where the "win" was added.
>
> Regards,
> Weimin
>
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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