You don't mention the condition, which is important (esp pH). Best pH values are 6-8.
From personal experience: try K2PtCl4 and OsCl3.
Try ~5 mM for 30-60' (quick soak).
The advantage of osmium salts is that they give a nice color to your crystals so you know something is binding.
HTH and GL, Bert
________________________________________
From: CCP4 bulletin board [[log in to unmask]] on behalf of RHYS GRINTER [[log in to unmask]]
Sent: Wednesday, January 15, 2014 5:18 PM
To: [log in to unmask]
Subject: [ccp4bb] Best compounds for heavy atom soaks
Hello message board,
My group has some crystals of an interesting protein to take to the synchrotron in a couple of weeks. We won't be able to prepare and crystallise a SelMet derivative during that time period, but we have loads of crystals sitting around. The diffraction isn't great, we see maybe 3.5 at home but might be enough to get over the line.
It will be a very difficult MR target, so we were thinking of soaking so crystals with heavy atomic compounds that we have lying around. I was wondering if people had any suggestions of compounds that people have used successfully for experimental phasing and maybe concentrations to use and soaking time.
Cheers,
Rhys
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