Did anybody mention native gel electrophoresis to select suitable HA ions?
Worked for us really nicely in a situation were speed was essential.
Here's a reference: PMID 14646083
Klaus
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Dr. Klaus Fütterer
Room 717, Biosciences Tower
School of Biosciences P: +44-(0)-121-414 5895
University of Birmingham F: +44-(0)-121-414 5925
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Birmingham, B15 2TT, UK W: http://tinyurl.com/futterer-lab
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On 15 Jan 2014, at 20:49, Matthew Franklin wrote:
> Hi Rhys -
>
> Don't forget to try sulfur-SAD, especially the multi-crystal version published recently:
>
> http://journals.iucr.org/d/issues/2013/07/00/ba5189/index.html
>
> This seems well suited to your situation.
>
> - Matt
>
>
> On 1/15/14 12:18 PM, RHYS GRINTER wrote:
>> Hello message board,
>>
>> My group has some crystals of an interesting protein to take to the synchrotron in a couple of weeks. We won't be able to prepare and crystallise a SelMet derivative during that time period, but we have loads of crystals sitting around. The diffraction isn't great, we see maybe 3.5 at home but might be enough to get over the line.
>> It will be a very difficult MR target, so we were thinking of soaking so crystals with heavy atomic compounds that we have lying around. I was wondering if people had any suggestions of compounds that people have used successfully for experimental phasing and maybe concentrations to use and soaking time.
>>
>> Cheers,
>>
>> Rhys
>>
>
>
> --
> Matthew Franklin, Ph. D.
> Senior Scientist
> New York Structural Biology Center
> 89 Convent Avenue, New York, NY 10027
> (212) 939-0660 ext. 9374
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