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Dear Klaus,
this list seems to be missing means to reduce the growth rate, in
particular micro seeding applied to a matrix screen of protein vs.
precipitant concentration:
Find the condition(s) just below crystals form and micro-seed with a
cat whisker!
Best,
Tim
On 01/22/2014 04:01 PM, Klaus Ftterer wrote:
> Dear ccp4bb contributors,
>
> We are dealing with the problem of a protein (~ 50 kDa) that
> crystallises readily, but has an annoying habit of forming highly
> intergrown rods or needles. Even when the crystals look optically
> homogenous under the microcsope, diffraction is so so (3.5 ᅤ or so
> on the synchrotron), but patterns reflect several crystal lattices
> that the processing software cannot resolve properly.
>
> We have tried this:
>
> - additive screens - switching the His-tag from N- to C-terminus -
> cutting the tag - thermal stability screens in a variety of
> buffers - growth in the presence of potential ligands/substrates
>
> Any suggestions for tricks that we haven't thought of so far?
>
> Thank you.
>
> Klaus
>
>
> =======================================================================
>
> Dr. Klaus Ftterer
>
> School of Biosciences P: +44-(0)-121-414 5895 University of
> Birmingham F: +44-(0)-121-414 5925 Edgbaston
> E: [log in to unmask] Birmingham, B15 2TT, UK W:
> http://tinyurl.com/futterer-lab
> =======================================================================
>
>
>
>
>
>
>
- --
- --
Dr Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen
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