Dear Matthias,
Have a look at this paper:
Large scale expression and purification of the mouse 5-HT3 receptor.
Hassaine G, Deluz C, Li XD, Graff A, Vogel H, Nury H.
Biochim Biophys Acta. 2013 Nov;1828(11):2544-52.
Best, xiaodan
-----Original Message-----
From: CCP4 bulletin board on behalf of Matthias Zebisch
Sent: Tue 1/7/2014 10:33
To: [log in to unmask]
Subject: [ccp4bb] Membrane protein structures from mammalian expression
Dear crystallographers,
happy new year to everybody!
Can I ask you to send me examples where expression of fully integral membrane proteins in mammalian cells (not insect!)
has led to the successfull determination of a crystal structure?
Thanks very much,
Matthias
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Dr. Matthias Zebisch
Division of Structural Biology,
Wellcome Trust Centre for Human Genetics,
University of Oxford,
Roosevelt Drive,
Oxford OX3 7BN, UK
Phone (+44) 1865 287549;
Fax (+44) 1865 287547
Email [log in to unmask]
Website http://www.strubi.ox.ac.uk
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