Hi Matthias,
relion_reconstruct will only use each particle in its optimal
orientations, whereas inside relion_refine a probability-weighted
angular assignment is calculated. That is what probably makes for this
difference (we see it all the time).
HTH, S
On 12/04/2013 03:35 PM, Brunner,Matthias wrote:
> Hi,
>
> is there a difference in how reconstruction is implemented in relion_reconstruct vs. relion_refine.
>
> The unfiltered reconstruction by the former (same _data.star file for both!) looks slightly worse. After map sharpening, the difference is quite striking. I did use the --ctf option for relion_reconstruct.
>
>
> Has anyone else ever experienced this?
>
>
> Version: RELION 1.2
>
>
> Best
--
Sjors Scheres
MRC Laboratory of Molecular Biology
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Cambridge CB2 0QH, U.K.
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