Hi Dieter,
If you use the output data.star from the 2D as input for the 3D, then
the answer to both your questions is yes.
HTH, S
On 12/09/2013 02:04 PM, Dieter Blaas wrote:
> Dear Sjors and all,
> suppose the coordinates of the particles in the micrographs are
> not very exact requiring an offset of, say 10 px for correct alignment
> during the 2D run. Will the (improved) coordinates (particle
> positions) then be taken over from the star file and used in the 3D
> run? If so, could one even reduce the offset in the following 3D run
> to, say 3 px? Clearly, this will depend from the data set but is this
> principally correct?
> Thanks a lot, Dieter
>
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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