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Dear Junyu,
You can test cryo conditions conveniently in the absence of your
crystals if you have access to e.g. an inhouse machine. Prepare your
solutions with the usual suspects (PEG400; PEG400+glycerol;
butanediol; Na Malonate etc. pp.) at various concentrations, pick up a
large as possible drop with a nylon loop and take a shot to see if you
have ice rings.
Try to be on the safe side with respect to the decision about the
minimal concentration because the presence of the crystal might give a
slightly different result.
Best,
Tim
On 12/16/2013 10:36 PM, Xiao, Junyu wrote:
> Dear all, sorry if this topic does not interest you. I wonder
> whether anyone has experience with freezing crystals grown in ~0.2
> M Magnesium Formate. Garman and Mitchell suggested that "A major
> anomaly is solution 44, 0.2 M magnesium formate, which requires 50%
> glycerol for cryoprotection" in their 1996 paper (J Appl. Cryst.
> 29, 584-587). Since 50% glycerol is kind of harsh, I wonder
> whether anyone has tried alternative cryo protectant. Your kind
> help will be highly appreciated.
>
> Best regards, Junyu
>
> ----------------------------------------------------------- Junyu
> Xiao, Ph.D. University of California, San Diego Leichtag Room 283
> 9500 Gilman Drive, 0721 La Jolla, CA 92093-0721 Lab phone:
> 858-822-0684
> ------------------------------------------------------------
>
>
- --
- --
Dr Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen
GPG Key ID = A46BEE1A
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