The kinase domain of FAK can be made constitutively active by a mutation
and expressed in E. coli. It's called "Super FAK Kinase". But i am afraid
it is rather specific. If you don't get a better lead I can send you the
sequence and a reference.
Gloria Borgstahl wrote:
> Does anyone know of a good way to phosphorylate the Tyr on a protein for structural
> studies? Is there a generic kinase that can be coexpressed or purified for phosphorylation?
>
> The pCMF Amber codon system is very expensive
> and Glu really doesn't mimic pTyr all that well.
>
> Any ideas/help would be appreciated, G
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