Dear CCP4ers
I have a data set that diffracts 1.96 Å. It scales in P21 Space group with 7% linear Rfactor.
The Mattew coefficient gives 10% probability for 3 molecules in the asymmetric unit, 53% for 4 molecules, and 36% for 5 molecules.
Molecular replacement just finds 3 molecules in the asymmetric unit. Running Phaser also gives a partial solution with 3 molecules.
When I refine just the back bone of the protein for the 3 molecules the Rfree/Rwork does not go better than 34% / 31%, and when I run the molecular replacement on the refined structure again, and I fix it as a model to search for another molecule, it still does not find it.
I have attached a photo to show the density for the fourth molecule in the asymmetric unit.
What is the solution to this?
Thanks in advance for your help
Meisam
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