Hi,
I am working with a protein which can assume different oligomerization forms, starting from monomers to trimers and even penta-decamers. We conformed this by Native PAGE and HPLC studies. The protein's theoretical monomeric molecular weight is 14.6 KDa (pI - 5.9) and it has some 140 amino acids with high Glutamic acid (24), Lysine (10) and Arginine (13) content. I have tried to crystallize it but not getting any hit as far now.
Previous study showed that this protein gets some stability by Calcium ion. With the calcium chloride conditions, I am getting spherical shaped structures, but not sure what are they; calcium chloride crystals or protein crystals. Can protein crystals be spherical in shape, specially when the protein behaves like an oligomer?
Also please let me know what is the minimum protein concentration required to obtain crystal for such small protein (if there is any empirical rule/idea).
Any suggestion will be highly appreciated.
Thanks and regards,
Debasish Kumar Ghosh
CSIR- Junior Research Fellow (PhD Scholar)
C/o: Dr. Akash Ranjan
Computational and Functional Genomics Group
Centre for DNA Fingerprinting and Diagnostics
Hyderabad, INDIA
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Telephone: 0091-9088787619 (M), 0091-40-24749396 (Lab)
Lab URL: http://www.cdfd.org.in/labpages/computational_functional_genomics.html
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