Dear all,
I am a new starter in the membrane protein field and working with the
cell free expression system. As the really small amount of protein I
could get in hand, I got somehow stuck in the quantification of the
protein yield. so Is there any suggestion of any methods or reagents
suitable for quantifying small amount of membrane protein with
detergents or lipids? Before, I used spectrum meter to see Abs280 while
the reaction was scaled up to 200ul, but mostly I was playing around
25-50ul reactions and could only detected my proteins from western blot.
Many thanks!
Kun Yu
PhD Student at CSSB-FZJ, DESY
Notkestr. 85,
22607 Hamburg, Germany
Tel.: +49 40 8998 6032
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