Hey David,
We also using Analysis and Cyana and have problems with export/import
from time to time. Unfortunatelly, most of the times it's because the
user misses one correct setting...
So maybe this helps.
to your problem 1:
Maybe the spectrum is not associated with the shift list. We always
make one "export" shift list to which we associate only the spectra
with the relevant shifts and all the accociated spectra have only one
peak list in order to avoid averaging.
In the Experiments:Spectra tab there is also an option for the shift
weighting for each spectra. In the Tolerances tab you can set the
weight for each dimension, e.g. if you set this to zero for the carbon
dimension, the shifts will not be exported.
to your problem 2:
We experienced something similar. We imported a shift list from cyana,
which was correctly imported as we looked in the resonance tab. But
when we exported this shift list again some Leu and Val methyl shifts
were swaped. After we assigned the shifts manually in the prot file
and imported it again the afterwards exported prot file was correct.
We haven't experienced it again so far. I don't know what happend
there but I would be interested to know whats causing this.
cheers,
Nina
Zitat von David Langelaan <[log in to unmask]>:
> Hello,
>
> I am having an issue exporting my shiftlist and noesy peak lists in
> xeasy format for future use in CYANA. There is no actual program
> crash, but the behaviour seems to be off (unless I am missing some
> type of setting).
>
> Here is the description of what I am doing:
>
> Using format converter I go and export in xeasy format. I first
> export the shiftlist, using the cyana format.
>
> I then go to the peaks export menu, and export my peaklist as cyana
> format, not using the original resonance names (this way resonances
> are given the number they are assigned in the shiftlist).
>
> At this point looking at the peak lists and shift lists there are a
> few issues.
> 1) For the aromatic noesy peak lists, although in my project my
> peaks are fully assigned, the carbon assignment is missing in the
> export lists.
>
> 2) This is a bit tricker, it seems that for my leucines, that the
> CD1/CD2 and HD1 and HD2 carbons and protons are being swapped.
> As an example:
>
> Here is peak 231 from my peak list:
> 231 3.492 62.361 0.889 1 T 1.534e+05 0.00e+00 a 0
> 2018 2017 993 0
>
> It is assigned to resonances 2018, 2017 and 993
>
> The relevant resonances from my shift list:
>
> 993 0.803 0.009 QD1 1742
> 2017 62.379 0.043 CA 1803
> 2018 3.487 0.010 HA 1803
> 994 0.879 0.009 QD2 1742
>
> As you can see, it looks like the indirect dimension assignment has
> been 'swapped' from 994 (what it should be, and what it is in the
> project) to 993. This happens for many peaks, and even for
> intramolecular peaks where both the CD and HD resonances are swapped.
>
> Also note: My project has 2 chains in it, (protein+peptide). I do
> not see this behaviour for the peptide, only for the protein
> assignments.
>
> I am running analysis 2.3, updated about a week ago.
>
> Thanks! Let me know if there is any thing that I am setting wrong,
> or if there is some way I can help troubleshoot this.
>
> David
>
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