Dear Zhizhi,
we had a case like that. I would switch to slightly different buffer
(e.g. different pH) so crystals do not appear overnight, and then do
crystallisation screening. I bet you will have many hits in different
conditions, likely with bigger crystals.
Good luck!
Best wishes,
Tomas
On Fri, Aug 9, 2013 at 8:31 PM, ZHIZHI WANG <[log in to unmask]> wrote:
> Hi all,
> After I purified my target protein by ion exchange, I left the fractions with high protein concentrations overnight @4C. Now I saw a lot of small needle crystals inside the EP tubes this morning.
> I wonder whether there is any technique or method to get bigger crystals from this?
>
> ZZ
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