A trick I like is just to freeze the reservoir solution or would-be cryo-solution without a crystal present. If the frozen solution stays clear and does not show ice rings on e.g. a home source, it is worth trying. Otherwise, the solution needs optimization.
Herman
-----Ursprüngliche Nachricht-----
Von: CCP4 bulletin board [mailto:[log in to unmask]] Im Auftrag von Uday Kumar
Gesendet: Freitag, 23. August 2013 19:52
An: [log in to unmask]
Betreff: [ccp4bb] cryoprotection
Hello
Can anyone suggest a cryoprotectant for the following crystallization condition
0.2-0.4M sodium formate
~20% PEG 3350
0-25 mM Nickel
0-100 mM Malonate
Thank you
with regards
uday
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