I've had good success transferring crystals grown in
200 mM NaOAc pH 5.5 / 10% PEG
Into a solution for room temperature diffraction (in ~6 steps, peg first, then buffers) :
100 mM NaOAc / 100 mM Malonate / 30% PEG
And from there into a cryo solution in 4-5 steps) that was:
100 mM NaOAc / 100 mM Malonate / 30% PEG / 20% Ethylene Glycol
Crystals have been solved to at least 1.4A resolution with mosaicity in the 0.2-0.3 range.
-Alex
On Aug 23, 2013, at 10:52 AM, Uday Kumar <[log in to unmask]> wrote:
> Hello
>
> Can anyone suggest a cryoprotectant for the following crystallization condition
>
> 0.2-0.4M sodium formate
>
> ~20% PEG 3350
>
> 0-25 mM Nickel
>
> 0-100 mM Malonate
>
> Thank you
>
> with regards
> uday
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