Dear BB, sorry for the off-topic question.
I am considering purifying a protein with a 3xFlag tag, obviously not the ideal option for obtaining large amounts for structural studies but nonetheless. I was wondering what experience people had with different purifications resins or columns in terms of price, efficiency, and purity (perhaps elution?). Any advice would be welcome. If people write to me personally I will gladly give an overview of all answers.
Thanks in advance,
Arnau
Arnau Casaņas, PhD
Institute of Molecular Biology and Biophysics
ETH Zurich
Schafmattstr. 20 HPK H10
8093 Zurich
Switzerland
+41.44.633845
|