Would you happen to know what the A280 of a 1M (or .1 M) solution is?
I wonder if this absorbance is really due to impurities or is the tail of a weak absorbance band of imidazole itself. I
notice A280 is not one of the properties sigma lists for its imidazole.
Jan wrote:
> Hi Bernhard,
> this one works for us:
> Sigma BioUltra imidazole, >99.5% by GC, 56749
>
> Cheers,
> Jan
> --
> Jan Abendroth
> Emerald BioStructures
> Seattle / Bainbridge Island WA, USA
> home: Jan.Abendroth_at_gmail.com
> work: JAbendroth_at_embios.com
> http://www.emeraldbiostructures.com
>
> On Aug 21, 2013, at 7:33 AM, Bernhard Rupp <[log in to unmask] <mailto:[log in to unmask]>> wrote:
>
>> Hi Fellows,
>> could someone please point me towards the source of a known high purity imidazole
>> with low absorbance at 280 nm? I am facing the problem of detecting a low absorption protein
>> in high imidazole background after IMAC gradient elution. In the UV spectra of the
>> 2 imidazoles I checked there is some contaminant that absorbs at 280…
>> Thx, BR
>> ----------------------------------------------------------------------------------------
>> Bernhard Rupp
>> Marie Curie Incoming International Fellow
>> Innsbruck Medical University
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>> A 6020 Innsbruck – Austria
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