At Elspeth's request, here's a link to a picture of the rack we use for
drying out shipping dewars.
https://www.dropbox.com/s/8ber2iikra9x73y/2013-02-21%2013.39.38.JPG
Very simple and both we and the Lea group here have found it to make a
huge difference the the effectiveness and longevity of our dewars.
Ed.
--
Dr. E.D. Lowe
Department of Biochemistry
University of Oxford
South Parks Road
Oxford, UK
OX1 3QU
e:[log in to unmask]
t: +44 (0) 1865 613288
f: +44 (0) 1865 613201
On 12/07/2013 14:27, "Edward Snell" <[log in to unmask]> wrote:
>Ditto - I was always impressed with the contraption in the Garman lab
>which, if I remember correctly, is made of a thick block of wood and some
>plumbing pipes. It is designed to hold empty open Dewars inverted so they
>could dry.
>
>Edward Snell Ph.D.
>Assistant Prof. Department of Structural Biology, SUNY Buffalo,
>Senior Scientist, Hauptman-Woodward Medical Research Institute
>700 Ellicott Street, Buffalo, NY 14203-1102
>Phone: (716) 898 8631 Fax: (716) 898 8660
>Skype: eddie.snell Email: [log in to unmask]
>Telepathy: 42.2 GHz
>
>Heisenberg was probably here!
>
>
>-----Original Message-----
>From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of
>Ginell, Stephan L.
>Sent: Friday, July 12, 2013 12:45 AM
>To: [log in to unmask]
>Subject: Re: [ccp4bb] frosted crystals during storage in pucks
>
>My experience with xtals frosting in LN2 either in a dewar, while
>freezing, or in pucks, has been because the LN2 was contaminated with ice
>crystals The fog you see above your dewar when freezing xtals is frozen
>water vapor...it will fall and collect in the LN2 and also deposit on the
>xtals. Dewars filled with recycled LN2 get contaminated with ice. Dewars
>dried upside down allow the cold gas to flow out and warm moist air to
>flow in and the water to condense inside the dewar (basic physics). To
>dry shipping dewars keep up right while warming.
>Steve
>
>Sent from my iPad
>
>On Jul 11, 2013, at 5:25 PM, "Nathaniel Clark"
><[log in to unmask]<mailto:[log in to unmask]>> wrote:
>
>At our last synchrotron trip, the beamline staff suggested that the
>problem was due to moisture accumulation in the dry shipper. They
>recommended storing them inverted (for a few weeks, if I recall), and/or
>putting a supply of dry air in the dewer. Haven't tried it yet!
>Nat
>
>
>On Thu, Jul 11, 2013 at 5:08 PM, <Rain Field>
><[log in to unmask]<mailto:[log in to unmask]>> wrote:
>Hi All,
>We found if the crystals are storaged in pucks for 3-4 days in shipping
>dewar (with liquid nitrogen), they are almost frosted.
>Although I can wash them with liquid nitrogen, but it's not convenient to
>do that for each crystals.
>I doubt it's because the humid air in North West America.
>Does anyone has an idea how to avoid this?
>Thank you!
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