On Wed, 24 Jul 2013 16:36:17 +0200, Hena Dutta <[log in to unmask]> wrote:
> Hi,
> Can anyone tell, if there is any database containing the crystallization
> conditions of published structures? I want to see the conditions people
> have used for those proteins having some structural similarity. Any
> suggestion would be appreciated.
> Regards...
> Hena
You can use the reports in the PDB database:
1) Sequence search with your sequence
2) Report pull down menu -> select Crystallization
How is that going to help? Even a single amino acid change can change the
manner a protein
crystallizes! The precipitant concentration is affected by the protein
concentration.
The purity and purification procedure can also affect crystallization.
Enrico.
--
Enrico A. Stura D.Phil. (Oxon) , Tel: 33 (0)1 69 08 4302 Office
Room 19, Bat.152, Tel: 33 (0)1 69 08 9449 Lab
http://www-dsv.cea.fr/ibitecs/simopro/ltmb/cristallogenese
LTMB, SIMOPRO, IBiTec-S, CE Saclay, 91191 Gif-sur-Yvette, FRANCE
http://scholar.google.com/citations?hl=en&user=Kvm06WIoPAsC&pagesize=100&sortby=pubdate
http://www.chem.gla.ac.uk/protein/mirror/stura/index2.html
e-mail: [log in to unmask] Fax: 33 (0)1 69 08 90 71
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