Tim,
> I see, as of version 1.2 , Relion now outputs unfiltered half volumes
> (_half1_class001_unfil.mrc) but a /filtered /final volume(
> _class001.mrc). Is it possible to extract an /unfiltered /final volume
> from the Relion 1.2, 3d auto-refinement procedure?
just sum up the two half volumes, as outlined in http://www2.mrc-lmb.cam.ac.uk/relion/index.php/Analyse_results#Getting_higher_resolution_and_map_sharpening
with xmipp 2.4:
xmipp_convert_spi22ccp4 -i relion_it029_half1_class001_unfil.mrc -o half1.spi
xmipp_convert_spi22ccp4 -i relion_it029_half2_class001_unfil.mrc -o half2.spi
xmipp_operate -i half1.spi -plus half2.spi -o sumhalves.spi
Best
--
Matthias Brunner <[log in to unmask]>
PhD student, Marlovits Lab
-Research Institute of Molecular Pathology (IMP)
-Inst of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA)
Dr. Bohr-Gasse 3, A-1030 Vienna, Austria
Phone: +43 1 79044 4791
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