On Sunday, 09 June 2013, Theresa Hsu wrote:
> Dear all
>
> A question for the cross-trained members of this forum - for small sized proteins, is NMR better than crystallography in terms of data collection (having crystals in the first place) and data processing? How about membrane proteins?
A relevant study is the comparison by Yee et al (2005) JACS 127:16512.
<http://pubs.acs.org/doi/abs/10.1021/ja053565+>
They tried to solve 263 small proteins using both NMR and crystallography.
43 only worked for NMR
43 only worked for X-ray
21 could be solved either way
So you could say it was a toss-up, but consider that
- As the size gets larger, NMR becomes increasingly impractical
- 156 (60%) weren't solved by either NMR or crystallography.
What is the relative cost of the failed attempt?
Ethan
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