Similar result. Taking all defaults except #atoms, amt protein:
kDa #res #sites BjvtRatio
protein of interest:
LBL: 469 12 0.0153 (4 e-)
Ruppweb 4273 12 0.016 (edge + 1 ev)
Ruppweb 4273 12 0.013 (CuKa)
Hemoglobin protomer
LBL 32 2 0.0239 (4 e-)
Ruppweb 291 2 0.024 (edge + 1 ev)
Ruppweb 291 2 0.020 (CuKa)
James Holton wrote:
> I put together a little jiffy for feasibility of MAD experiments here:
> http://bl831.als.lbl.gov/xtalsize.html
>
> It does not calculate the f" value for you, but in general f" is ~4
> electrons for K edges and ~10 electrons for L edges. Crossec can give
> you more accurate values than this as long as you are "far away" from
> the absorption edge (10-100 eV), but if you are right on top of the
> "white line", then you have to measure f" anyway using an absorption
> scan. That said, it is very rare for the white line to be more than
> double the edge jump and Fe is not particularly spectacular for white
> lines, so I wouldn't expect much more than ~6 electrons from iron.
>
> -James Holton
> MAD Scientist
>
>
> On 6/2/2013 8:47 AM, Edward A. Berry wrote:
>> Is Ethan Merritt's anomalous scattering page at:
>> http://www.bmsc.washington.edu/scatter/
>> down or moved, or the firewall I'm behind is blocking it?
>>
>> I want to check feasibility of a native-iron MAD experiment,
>> and I'm not very good at math.
>>
>> thanks,
>> eab
>
|