Yes- this has happened to us so often recently that I've taken to checking every
new crystal form against the "nearest cell" server before starting heavy atom search.
That would account for the poor reproducibility.
Yeast glutamate-cysteine ligase (e.g. 3LVV) crystallizes with nearly identical cell
parameters to those given, but nothing else very close.
And i don't suppose one would be expressing a 3 kDa bacterial protein
in Saccharomyces. Peptide synthesis, more likely?
Jim Pflugrath wrote:
> Maybe you crystallized something else? Did you look up that unit cell in the PDB?
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> I am having a few issues with a data set I have been working on recently, and was hoping to get some ideas on how to
> deal with it, if anyone is in the mood.
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