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Dear Afshan,
- - Can you reproduce the crystals in the Linbro plates with the
identical solution used for the screening plate?
- - Did you compare the composition of the Linbro plate vs. the
screening plate?
- - Did you check e.g. the pH of the original solution?
- - Did you refresh the beta-ME / try a new protein prep? It goes off
after some time, as far as I know.
Regards,
Tim
On 04/16/2013 11:21 AM, Afshan Begum wrote:
>
>
> Dear All,
>
> I have encountered one problem to optimization the crystallization
> condition manually. Actually i got the good shape and even size
> crystal in a screening plate. The condition are :
>
> 20% PEG 6000, 0.1M MES pH 6.0 whereas protein in 10mM Na-acetate pH
> 5.7 contain 50mM NaCl, 1mM EDTA &5mM BME. When I tried to optimize
> this on Linbro the drops are become heavily precipitate even a
> mints. I tried so many things to avoid this ppt such as start PEG
> conc from 7%, dilute protein at half conc., add more salt in a
> reservoir solution but no succeeds. The protein prep is same which
> was using to get the Hits.
>
>
> I would be very thankful if you people give me nice suggestions.
>
> Thanks
>
>
> Best Regards
>
> AFSHAN
- --
- --
Dr Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen
GPG Key ID = A46BEE1A
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