Hi Afshan,
You mention:
The protein prep is same which was using to get the Hits.
So how old is your prep ? Was it stored at 4C or in LN2? Do you know if your protein is stable under the conditions you stored it ?
How long after purification did it take you to get those crystals ?
How old is your screen ? MES goes bad as well over time.
Jürgen
......................
Jürgen Bosch
Johns Hopkins Bloomberg School of Public Health
Department of Biochemistry & Molecular Biology
Johns Hopkins Malaria Research Institute
615 North Wolfe Street, W8708
Baltimore, MD 21205
Phone: +1-410-614-4742
Lab: +1-410-614-4894
Fax: +1-410-955-3655
http://lupo.jhsph.edu
On Apr 16, 2013, at 5:21, "Afshan Begum" <[log in to unmask]> wrote:
> The protein prep is same which was using to get the Hits.
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