Hi -
I'm working on a mammalian derived crystal. In a few chains where I can see the N-terminus, it appears that they are acetylated. I looked for previous discussion on how to deal with this, and I was wondering how exactly to deal with this in coot as of 2013.
Thus far, I added an ala to the n-terminus, then used
extensions>modelling>replace residue
to mutate to ACE
I'm running coot 0.7 stable release sept 2012.
I'm not seeing a bond between the original N-term N atom and the C of he acetyl group
Real Space Refine Zone and Regularize Zone do not like the setup.
I'm sure I need to define some things, but haven't dealt with this before, as for things like this, I used to switch to xfit.
Would it be better to ask at CCP4?
Thanks,
Dave
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